Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 21;10:78. doi: 10.3389/fnana.2016.00078

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Lenzi, Lazzeri, Biagioni, Busceti, Gambardella, Salvetti and Fornai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Confocal microscopy of autophagoproteasomes low magnification. Confocal microscopy confirms the occurrence of autophagoprotesome as shown by representative images at low magnification. At the highest dose of rapamycin the amount of P20S immunostaining (A) is scattered in the cell and only a few red puncta were observed (arrows). At this magnification the difference between P20S immunostaining and the intense LC3 immunostaining as shown by green puncta (arrows) is even more evident (B). Co-localization of P20S + LC3 within the same cells was shown by merging as orange/yellow puncta (arrows) (C). Again these representative pictures at low magnification indicate that, for the highest doses of rapamycin, P20S is mostly dragged down within LC3 positive vacuoles making it more compartmentalized than LC3 itself. Scale bar = 12 μm.