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. 2016 Jul 21;10:78. doi: 10.3389/fnana.2016.00078

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Copyright © 2016 Lenzi, Lazzeri, Biagioni, Busceti, Gambardella, Salvetti and Fornai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electron microscopy of autophagoproteasome in PC12 cells. The concomitant occurrence of P20S and LC3 immunostainig within a double membrane (thick arrows) vacuole is shown in representative micrograph. The LC3 staining is shown by small (10 nm) immunogold particles indicated by thin arrows while P20S staining is shown by large (20 nm) immunogold particles indicated by arrowheads. This confirms in a rat-derived cell line convergence between autophagy and proteasome in the novel clearing organelle autophagoproteasome. Scale bars = 75 nm.