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. 2015 Jul 25;9(5):394–405. doi: 10.1080/19336918.2015.1035504

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© 2015 Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — Monocyte chemoattractant protein 1 (MCP1) and haptoglobin (Hp) are affected in their capacity to migrate by CCR2 antagonist (RS1028959 exposition). Wound-healing assays were performed on SKOV-3 cells for 0, 12, 24 and 48 h at the indicated doses of Hp (250 μg) and monocyte chemoattractant protein 1 (MCP1, 250 ng). In addition, the effect of pretreatment with the chemokine (C-C motif) receptor 2 (CCR2) synthetic antagonist RS102895 (5 μM) on the capacity of SKOV-3 cells to migrate toward MCP1 (250 ng/ml) and Hp (0.250 mg/ml) is shown. As a negative control, bovine serum albumin (BSA) (1 mg/ml) was used. Chemotaxis was measured by confocal microscopy; nuclei (blue) were stained with DAPI (1:50). (B) Quantitative analysis of migration by the software Zen 2011 (Blue edition, Carl Zeiss) considering an average area of 9 × 10⁵ μm². This program determines the mean fluorescence intensity of each condition, which is plotted as a histogram. The results correspond to the average of 3 independent experiments. Bar scale = 100 μm.