Table 5.

Extraction via disposable pipettes yielded efficient recoveries with minimal matrix effects enabling sensitive limits of quantification (0.5-5.0 μg/L).

Analyte	Recovery (%, n=10)		Matrix Effect (%, n=10)
	Low	High	Low	High
THCa	59.9	58.7	−23.0	−21.5
11-OH-THCa	78.6	79.5	−6.1	−4.8
THCCOOHa	71.3	72.1	−17.5	−20.6
CBDa	73.0	73.6	−6.5	−6.1
CBNa	54.0	55.4	−11.0	−14.1
CBGb	63.5	65.5	−10.8	−13.0
THCVb	66.4	69.0	30.6	23.9
THCVCOOHb	70.1	75.3	7.7	3.5
THC-glucuronidea	71.3	72.2	4.5	1.1
THCCOOH-glucuronidec	55.4	56.4	2.2	−1.4
THC-d3	65.2	64.9	−25.8	−25.7
11-OH-THC-d3	84.4	83.2	−14.3	−10.4
THCCOOH-d9	75.2	75.5	−18.4	−18.6
CBD-d3	81.4	80.6	−13.7	−8.4
CBN-d3	59.2	59.7	−16.2	−15.9
THCCOOH-glucuronide-d3	58.8	59.3	−3.3	−5.5

THC Δ⁹-tetrahydrocannabinol, 11-OH-THC 11-hydroxy-THC, THCCOOH 11-nor-9-carboxy-THC, CBD cannabidiol, CBN cannabinol, CBG cannabigerol, THCV Δ⁹-tetrahydrocannabivarin, THCVCOOH 11-nor-9-carboxy-THCV

^aLow-quality control concentration for THC, 11-OH-THC, THCOOH, CBD, CBN, and THC-glucuronide was 1.5μg/L. High-quality control concentration for THC and THCOOH was 80μg/L and for 11-OH-THC, CBD, CBN, and THC-glucuronide it was 40μg/L

^bLow- and high-quality control concentrations for CBG, THCV, and THCVCOOH were 3 and 80μg/L, respectively

^cLow- and high-quality control concentrations for THCCOOH-glucuronide were 15 and 400μg/L, respectively