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. Author manuscript; available in PMC: 2017 Jul 7.
Published in final edited form as: Mol Cell. 2016 Jun 30;63(1):167–178. doi: 10.1016/j.molcel.2016.05.032

Figure 1. Distribution, frequency, and dynamics of RNA:DNA hybrids.

Figure 1

(A) Screenshot of a representative genomic region. DRIPc data is shown in red (+ strand) and blue (− strand) (two independent replicates). DRIP-seq data is in green; DRIP-seq data after RNase A and RNase H pre-treatment is shown below (in khaki and teal, respectively). (B) Distribution of DRIPc peak numbers as a function of peak size. (C) Bar chart of DRIP-qPCR (as % input) for a various loci. Each bar is the average of two independent experiments (shown with standard error). The effect of RNase A and RNase H pre-treatment on DRIP-qPCR is shown. (D) Location analysis of DRIPc peaks (right) compared to expected genomic distribution (left). The various regions are color-coded as per cartoon below. ‘X’ refers to an extended 3 kb region, as shown. (E) Number of DRIPc peaks in the sense and antisense orientations relative to gene transcription. (F) R-loops were measured by DRIP-qPCR at a number of loci through a time course post DRB treatment and wash (5’ indicates promoters; 3’ indicates terminators). The initial DRIP-qPCR value for each locus prior DRB treatment (time zero) was normalized to 100%. Bars represent the average of two independent experiments with standard error.