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. Author manuscript; available in PMC: 2016 Jul 21.
Published in final edited form as: Tissue Eng. 2002 Oct;8(5):771–785. doi: 10.1089/10763270260424132

FIG. 7.

FIG. 7

Phenotypic characteristics of BMSCs grown on LDI–glucose polymer or TCPS. (A) Hydroxyproline content of collagen type I synthesized by cells grown on polymer or on TCPS for 2 weeks. The experiment was performed as described in Materials and Methods. The results are presented as means ± standard deviation (n = 3). (B) Comparative assessment of collagen type I and TGF-β1 mRNA expression in cells grown on LDI–glucose polymer or TCPS for 7 or 14 days. The cells were seeded at equal density (0.2 × 106 cells/well) on polymer or TCPS, and grown under identical conditions. After incubation, cells were trypsinized and mRNA was extracted for analysis by RT-PCR. Expression of mRNA for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a housekeeping gene, was used as a control to show equivalent input of RNA in all lanes. The results shown were obtained from one of three individual experiments.