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. Author manuscript; available in PMC: 2016 Jul 21.
Published in final edited form as: Neurosci Lett. 2015 Sep 10;607:17–22. doi: 10.1016/j.neulet.2015.09.006

Fig. 2.

Fig. 2

Effects of 2 mM ketamine (internal exposure comparable to human anesthetic plasma concentrations) and ALCAR on 5-HT and 5-HIAA levels in zebrafish embryos.

Embryos at 52 hpf were treated with 2 mM ketamine, 2 mM ketamine + 0.5 mM ALCAR and 0.5 mM ALCAR alone. Twenty four hours post exposure 76 hpf embryos were processed for 5-HT (A) as well as its metabolites, 5-HIAA (B) measurements using HPLC/EC. Values are expressed in ng/μg protein in the embryo extract as mean ± SD. One-way ANOVA with post-hoc analyses were used to obtain statistical significance (*P < 0.05; **P < 0.001; ***P < 0.0001) of variation in the 5-HT and its metabolites, 5-HIAA levels between the experimental groups as well as with the control.