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Steady-state DNA polymerase efficiency with chimeric ATP-releasing nucleotides, with Kf exo−.
| dNTP |
kcat (min–1) |
Km (μM) |
kcat/Km (μM−1min−1) |
|---|---|---|---|
| dGTP | 15.4 ± 0.5 | 0.11 ± 0.01 | 140 |
| dGppppA | 7.1 ± 0.5 | 3.5 ± 0.4 | 2.0 |
| dCTP | 14.0 ± 0.4 | 0.07 ± 0.01 | 200 |
| dCppppA | 12.9 ± 0.1 | 3.0 ± 0.2 | 4.3 |
| dATP | 8.6 ± 0.3 | 0.35 ± 0.05 | 25 |
| dAppppA | 7.1 ± 0.2 | 1.3 ± 0.4 | 5.5 |
| dTTP | 8.7 ± 0.3 | 0.24 ± 0.06 | 36 |
| dTppppA | 3.7 ± 0.1 | 2.2 ± 0.6 | 1.7 |
