Table 1.
Baseline Values of Viability of Jurkat Cells Cryopreserved with 10% DMSO and Stored in LN2 Assessed by Various Methods
| Viability (%) of cells cryopreserved in 10% DMSO | |||
|---|---|---|---|
| Method | HV | IV | LV |
| Trypan Blue | 80.0 | 48.1 | 5.8 |
| CASY | 82.8 | 68.2 | 65.1 |
| Flow cytometry | 67.8 | 24.8 | 5.0 |
| Viability (%) of cells cryopreserved in CryoStor 10 | |||
|---|---|---|---|
| Method | HV | IV | LV |
| Trypan Blue | 86.1 | 48.4 | 12.7 |
| CASY | 85.2 | 77.5 | 68.5 |
| Flow cytometry | 78.5 | 29.7 | 6.3 |
Initial cell suspension quality before storage was set as: HV=high viability, IV=intermediate viability, LV=low viability.