Table 3.

Effects of Cryopreservation Media and Temperature Conditions on IFN-γ ELISPOT Responses of Cell Suspensions of Different Initial Quality

A. PBMCs
ELISPOT Results (mean spots/10^6 cells)
Sample Type	Storage media	Temp condition	CEF	PHA
HV PBMC	Cryostor 10	Stored LN2	564	tmtc
HV PBMC	Cryostor 10	Shipped LN2	643	tmtc
HV PBMC	10% DMSO +FBS	Stored LN2	614	tmtc
HV PBMC	10% DMSO+FBS	Shipped LN2	433	tmtc
HV PBMC	AQIX	Stored RT 1d	703	tmtc
HV PBMC	AQIX	Stored RT 2d	663	tmtc
IV PBMC	Cryostor 10	Stored LN2	13	541
IV PBMC	Cryostor 10	Shipped LN2	10	649
IV PBMC	10% DMSO+FBS	Stored LN2	18	543
IV PBMC	10% DMSO+FBS	Shipped LN2	6	570
IV PBMC	AQIX	Stored RT 1d	11	473
IV PBMC	AQIX	Stored RT 2d	28	393

B. Jurkat cells
ELISPOT Results (mean spots/10^6 cells)
Sample type	Storage media	Temp condition	CEF	PHA
IV Jurkat	Cryostor 10	Shipped LN2	1	215
IV Jurkat	Cryostor 10	Shipped Dry Ice	1	229
IV Jurkat	10% DMSO+FBS	Shipped LN2	0	139
IV Jurkat	10% DMSO+FBS	Shipped Dry Ice	0	183
IV Jurkat	AQIX	Shipped RT	1	31
LV Jurkat	Cryostor 10	Shipped LN2	QNS	43
LV Jurkat	Cryostor 10	Shipped Dry Ice	0	10
LV Jurkat	10% DMSO+FBS	Shipped LN2	QNS	28
LV Jurkat	10% DMSO+FBS	Shipped Dry Ice	QNS	13

HV=high viability, IV=intermediate viability, LV=low viability; A. PBMCs, B. Jurkat cells;

QNS, quantity not sufficient; tmtc, too many to count.