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. Author manuscript; available in PMC: 2016 Jul 21.
Published in final edited form as: Nat Med. 2015 Nov 2;21(12):1445–1454. doi: 10.1038/nm.3982

Figure 3. AKT3 kinase activity is essential for aberrant migration phenotype.

Figure 3

(a) In utero electroporation at E14.5 of GFP vector encoding AKT3 variants, harvested at E18.5. Images were inverted for visibility, co-stained with phospho-S6 (pS6) of boxed regions below, enhanced contrast for visibility. (b) Localization of GFP+ cells quantified in upper (uCP); middle (mCP), and lower cortical plate (loCP). (c) Quantification of pS6+GFP+ pixels for the images described in (a). Values: mean ± s.d. (n = 3, 3, 6, 5 and 5 for each condition). n.s., not significant; ***, P < 0.001, G-test of goodness-of-fit (b); Student’s t-test (c). Scale bars: 100 μm.