Table 1.

Peak assignment for SERS spectra of RBCs and iRBCs at different infection time.

Observed Raman Shift Δν (cm−1)	RBC	ring stage iRBC (16h)	ring stage iRBC (24h)	late stage iRBC (32h)	Vibrational Mode Assignment
421	s	s	m	vw	Cholesterol
520	s	s	m	w	S-S disulfide stretching in proteins
650			w	s	C-C twisting mode of tyrosine
723	vw	vw	vw	vs	characteristic for phospholipids, lipid assignment
748	w	w	m	w	Symmetric breathing of tryptophan (protein assignment)
840	vs	vs	s	w	Glucose-saccharide band
951	s	s	vs	vs	ν3(CH3) of proteins
1087	m	m	m	s	${\nu }_1\mathrm{C}{\mathrm{O}}_3^{2-},{\nu }_3\mathrm{P}{\mathrm{O}}_4^{3-}$ ν(C- C) skeletal of acyl backbone in lipid
1124	vs	s	m	m	Proteins, lipids: C–N and C–C stretch
1247	m	m	m	m	Amide III (collagen assignment)
1373	m	m	s	s	The most pronounced saccharide band
1554				m	ν(C=C), tryptophan (protein assignment), ν(C=C), porphyrin
1599		vs	vs	w	Amide I band of proteins-Due to C=O stretching
1712				m	ν(C=O)OH (amino acids aspartic & glutamic acid)

^*Note the abbreviation represents the peak strength: vw – very weak, w – weak, m – medium, s – strong, vs – very strong.