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. Author manuscript; available in PMC: 2016 Dec 27.
Published in final edited form as: Nat Chem Biol. 2016 Jun 27;12(8):641–647. doi: 10.1038/nchembio.2113

Figure 4. CL but not MLCL is associated with proteins.

Figure 4

(a) Mitochondrial membranes were prepared from human lymphoblasts and solubilized with SDS. 31P-NMR spectra show resonances of all major and some minor phospholipids. CL was detectable in control samples; CL and MLCL were detectable in BTHS samples. (b–c) Mitochondrial membranes, prepared from human lymphoblasts (b) or mouse kidneys (c), were solubilized with digitonin. 31P-NMR spectra show the major phospholipids with broader resonances compared to the SDS experiment. MLCL (chemical shift values of MLCL standard: 1.0, 1.2 ppm) was detectable in tafazzin-deficient samples but CL was not.