Figure 2. Experimental protocol schematic.
For all experiments after the pilot MRI study, neuroinflammatory insult was administered at time (t)=0, IV ferumoxytol or saline (control) was administered at t=24 h, and MRI was performed at t=48 h. Immediately after MRI brains were extracted for immunohistochemistry (IHC) analysis, immunofluorescent histochemistry (IFHC) analysis, and electron microscopy (EM) analysis.