Figure 2.

Defect in positive selection of TCR transgenic CD8+ T cells in 4KO thymi. Gating of (a) Rag1^−/− OT-I → WT or 4KO BM chimeras and (f) Tcra^−/− P14 → wild type or 4KO BM chimeras. Number of (b) CD4^-CD8⁺V_α2^hi or (c) CD4⁺CD8^lo thymocytes, (d) V_α2 GMFI on CD4⁺CD8^lo thymocytes, and (e) histograms of V_α2 on CD4⁺CD8^lo thymocytes in mice receiving Rag1^−/− OT-I BM (n=9 wild-type, 16 4KO, male and female, combined from 2 experiments). Number of (g) CD4^–CD8⁺V_α2^hi or (h) CD4⁺CD8^lo thymocytes, (i) V_α2 GMFI on CD4⁺CD8^loV_α2⁺ thymocytes, and (j) histograms of V_α2 on CD4⁺CD8^loV_α2⁺ thymocytes in mice receiving Tcra^–/P14 BM (n=14 wild-type, 15 4KO, male and female, combined from 2 experiments). *P <0.0245 **P <0.0068 ***P <0.0056 ****P <0.0025 *****P <0.0001 in b-d, g and h (Student's t-test mean±SD)