Fig 3.

CD8 lineage cells express CD69 and TCRbeta in 4KO mice. Flow cytometric plots (a) and quantitation (b) of CD69⁺TCRβ^intCD103⁺CD4⁺CD8α^lo thymocytes in wild-type, 4KO, or B2m^−/− mice (n=4 mice each group, male and female, combined from 2 experiments). (c) Quantitation of CD69⁺TCRβ^intCD103⁺CD4⁺CD8β^lo thymocytes in wild-type or 4KO mice (n=9 wild-type, 8 4KO mice, male and female, combined from 2 experiments) (Numbers on gates indicate percent of total thymocytes.) *P<0.05 and NS, not significant in b (One-way ANOVA with Dunnett's multiple comparison post-test). NS in c (Student's t-test) b and c (mean±SD)