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Hes1 represses Cxcl1 transcription without altering NF-κB and MAPK activation. (a) Immunoblot analysis of the indicated proteins in whole cell lysates of LPS-treated BMDMs from WT and Hes1 KO paired littermates. (b,c) Levels of primary transcripts of Cxcl1 (b) and Tnf (c) in BMDMs from WT and Hes1 KO littermates were quantitated by qPCR. Data are shown as mean and s.d. of triplicate determinants.
