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. Author manuscript; available in PMC: 2016 Dec 20.
Published in final edited form as: Nat Immunol. 2016 Jun 20;17(8):930–937. doi: 10.1038/ni.3486

Figure 7.

Figure 7

Hes1 inhibits Cxcl1 productive elongation by suppressing recruitment of the P-TEFb complex. ChIP assays showing the occupancy of S2P Pol II (a-b, left panels) and CDK9 (c-e, left panels) at the TSS regions and gene body regions of Cxcl1 (a-d) and Tnf (e) in Hes1+/+Mx1-Cre (WT) and Hes1fl/flMx1-Cre (Hes1 KO) BMDMs untreated or stimulated with LPS (10 ng/ml) for 1 h. Cumulative results from three independent experiments were shown for S2P Pol II (a-b, right panels) and CDK9 (c-e, right panels) on the Cxcl1 and Tnf gene loci under LPS-stimulated conditions and relative occupancy in WT cells was set to 1. (f) CDK9 ChIP-seq and Hes1 ChIP-seq gene tracks show CDK9 (upper) and Hes1 (lower) distribution along the Cxcl1 locus in BMDMs untreated or stimulated with LPS (10 ng/ml) for 1 h. Red box, TSS region of Cxcl1. *P<0.05 (Student's t-test).