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. Author manuscript; available in PMC: 2017 Jul 19.
Published in final edited form as: Mol Biosyst. 2016 Jul 19;12(8):2471–2480. doi: 10.1039/c6mb00175k

Fig. 2. Protein glutathionylation is highly dependent on glucose metabolism in the presence of the electron transport chain blocker.

Fig. 2

After incubation of azido-Ala to HEK293/GS M4 cells for 20 h, cells were subjected to the following stimulus: (A) glucose starvation with antimycin A in a time-dependent manner, (B) glucose starvation with an increasing concentration of antimycin A, (C) decreasing glucose concentrations with antimycin A, and (D) increasing incubation time of antimycin A in a physiological glucose concentration (5 mM). After stimulus, cell lysates were subjected to click reaction with rhodamine-alkyne, and analyzed for fluorescence and Coomassie stain.