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. Author manuscript; available in PMC: 2017 Jul 19.
Published in final edited form as: Mol Biosyst. 2016 Jul 19;12(8):2471–2480. doi: 10.1039/c6mb00175k

Fig. 4. Proteomic analysis of glutathionylated proteins.

Fig. 4

(A) Approach for pull-down and mass analysis: after click reaction with biotin-alkyne, glutathionylated proteins enriched by streptavidin-agarose were eluted for silver staining or directly digested on-beads for LC-MS/MS analysis. (B) Analysis of enriched proteins in indicated conditions. (C) The number of glutathionylated proteins in indicated conditions by LC-MS/MS. ‘Positive’ and ‘negative’ indicate proteins enriched in lane 4 and 1, respectively, in Figure 4B. (D) DAVID gene ontology (GO) analysis of identified glutathionylated proteome. (E-F) Comparison of glutathionylated proteins in glucose starvation/antimycin A with previous proteomic data of glutathionylome (E) or sulfenylome (F) by treatment of exogenous H2O2.