Table 1.

Bacteria reverse mutation assay (Ames test) for Calabadion 2, in the presence or absence of rat liver S9 fraction (−/+S9) in order to test for metabolic activation. Displayed are numbers of colonies growing after treatment with the test compound (Data are ± SD) and the ratio of the number of colonies growing after treatment with the test compound relative to untreated bacteria.

	TA 1535		TA 1537		TA 98		TA 100
Calabadion 2 concentration (mg)	−S9	+S9	−S9	+ S9	−S9	+ S9	−S9	+ S9
0	11 ± 2	8 ± 2	10 ± 5	7 ± 1	19 ± 2	27 ± 3	100 ± 27	109 ± 4
0.012	6 ± 1	10 ± 2	7 ± 2	2 ± 1	24 ± 4	24 ± 4	92 ± 11	114 ± 2
0.037	10 ± 5	6 ± 2	8 ± 2	4 ± 1	18 ± 8	27 ± 3	100 ± 6	112 ± 8
0.11	8 ± 3	7 ± 1	7 ± 2	6 ± 1	30 ± 10	23 ± 5	94 ± 4	91 ± 6
0.33	7 ± 4	10 ± 5	8 ± 1	6 ± 1	21 ± 5	24 ± 7	105 ± 9	101 ± 7
1	8 ± 3	7 ± 3	3 ± 2	5 ± 2	20 ± 5	29 ± 6	106 ± 6	97 ± 4
Ratio	0.72	0.87	0.3	0.71	1.05	1.07	1.06	0.88
Positive Control	SA 446	2-AA 91	191-A 250	2-AA 141	D-myc 505	2-AA 1101	SA 282	2-AA 1307

191-A = ICR 191 Acridine; 2-AA = 2-Aminoanthracene; D-myc = Daunomycin; SA = Sodium Azide; TA 98-1535=Strain number of Salmonella thyphimurium