Evaluation of the mechanical response of the de novo ECM: A) seven protocol biaxial test data before (PEUU+ECM, blue dots) and after (dPEUU black dots) degradation of one representative sample (30% strain, 21 days), and fitted ψ-surfaces of construct, scaffold, and de novo ECM (blue, red and green respectively); B) stored energy density under equi-strain conditions; C) membrane tension response under equi-strain conditions, D) fiber ensemble stress vs. strain response; and E) rate of change of ensemble stress vs. ensemble strain (recruitment).