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. Author manuscript; available in PMC: 2016 Dec 27.
Published in final edited form as: Nat Immunol. 2016 Jun 27;17(8):946–955. doi: 10.1038/ni.3491

Figure 2.

Figure 2

Thymocytes expressing the YAe62β chain are biased to react with self-peptides presented by multiple haplotypes of MHC. (a) Pre-selection TCRβ+ DP thymocytes derived from 4–6 week old H2-Ab1−/− B2m−/− (MHC-deficient) YAe62β, C57BL/6 and B3K506β mice were cultured with BM-DC generated from C57BL/6 (H2b), NOD (H2g7), Balb/c (H2d), C57BL/6.H2-Ab1−/− (MHCII-deficient), C57BL/6.B2m−/− (MHCI-deficient), or C57BL/6.H2-Ab1−/−B2m−/− (MHC-deficient) mice, and analyzed for the expression of CD69 and Nur77-GFP. Representative dot plots are shown from two independent experiments. (b) Quantification of the frequency at which DP thymocytes express CD69 and Nur77-GFP following culture with BM-DC. (c) Nur77-GFP expression levels on activated (Nur77+CD69+) DP thymocytes. *P <0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (unpaired two-tailed Student’s t-test). Data are combined from two independent experiments for (n = 6) YAe62β, C57BL/6 and B3K506β mice (b,c mean ± s.e.m.).