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. Author manuscript; available in PMC: 2016 Dec 27.
Published in final edited form as: Nat Immunol. 2016 Jun 27;17(8):946–955. doi: 10.1038/ni.3491

Figure 5.

Figure 5

The identical CDR3β P6–7 doublets promote or limit pre-selection DP thymocytes to react with multiple haplotypes and alleles of MHC. (a–f) Sequence (a,b), quantification of the frequency at which CD4+CD8+ thymocytes express CD69 and Nur77-GFP (c,d), and Nur77-GFP expression levels on activated Nur77+CD69+ DP thymocytes isolated from TCRβ retrogenic mice (e,f) that express Vβ8.2-Jβ2.4 TCRs carrying the CDR3β P6–7 doublet, AW or EG, or Vβ8.2-Jβ2.7 TCRs carrying SW or EQ, following culture with BM-DC that express the H-2b, H-2g7, H-2s, H-2k, H-2q, H-2d, H2-Ab1−/−, B2m−/− or H2-Ab1−/− and B2m−/− (MHC-deficient) haplotype. *P <0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (unpaired two-tailed Student’s t-test). Data are combined from two independent experiments for (SW n = 6, AW, EQ, EG n = 5) mice (b–f, mean ± s.e.m.).