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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: J Immunol. 2016 Jun 20;197(3):726–735. doi: 10.4049/jimmunol.1600253

Table I.

Primers used for chromatin immunoprecipitation (ChIP)-qPCR

ChIP Antibody Promoter Site Forward primer Reverse primer Ta (°C)1
NFκB p65 IFN-γ 1 AGTGTGTGTCTAGTGCTGGA GTCAACGTGCCCAGAAAGAA 58
IL-10 1 ATTATGACCTGGGAGTGCGT TGTGGCTTTGGTAGTGCAAG 56
T-bet 1 GAGTTTCAGGTGGCAGGTTG TCCTGGGCTTTCTCTGCAG 58
Eomes 1 AGTCTCCCTCCTCTTCATACCT AGGCCATGAATTTGAGAGGGG 56
2 TAAGCCACGGAAAACCAGGC GGTGTCTCAGGCACACTTTAAAAT 56
3 GTCAGCCCGAGTTCTCTGAG AAGCTTTCCAACCTGGGTCC 56
T-bet / Eomes IFN-γ CNS-22 CCAGGACAGAGGTGTTAAGCCA GCAACTTCTTTCTTCTCAGGGTG 58
CNS-34 GGTATGCATCATCCCGGG TGGCCTGTCTTCAGAAGTTTGC 58
IL-10 1 GTTACACGTCTCCAAGGCTG GCAGTTGGTCAGAGGAGAGT 58
1

Ta is the annealing temperature used for PCR. CNS indicates conserved nucleotide sequence (across multiple species) in the interferon (IFN)-γ enhancer.