Table 1.
Primers used in the current study.
| Gene | Species | Forward sequence | Reverse sequence | Amplicon Size (bp) |
|---|---|---|---|---|
| Cxcl5 | Human | TCTGCAAGTGTTCGCCATAG | GAAACTTTTCCATGCGTGCT | 183 |
| Mouse | TGGCATTTCTGTTGCTGTTC | CACCTCCAAATTAGCGATCAA | 140 | |
| IL-6 | Human/Mouse | GGCTAAGGACCAAGACCATCCAA | TCTGACCACAGTGAGGAATGTCCA | 138 |
| IL-8 | Human | GGCCGTGGCTCTCTTGGCAG | TGTGTTGGCGCAGTGTGGTCC | 178 |
| IL-1β | Human/Mouse | AGTGTGGATCCCAAGCAATACCCA | TGTCCTGACCACTGTTGTTTCCCA | 175 |
| iNOS | Human | AATGAGTCCCCGCAGCCCCT | AGTCATCCCGCTGCCCCAGT | 143 |
| Mouse | CTGCTGGTGGTGACAAGCACATTT | ATGTCATGAGCAAAGGCGCAGAAC | 167 | |
| KC (Cxcl1) | Mouse | GCTGGGATTCACCTCAAGAA | TCTCCGTTACTTGGGGACAC | 180 |
| PCNA | Mouse | AAAGATGCCGTCGGGTGAATTTGC | AATGTTCCCATTGCCAAGCTCTCC | 130 |
| Tg- TLR4 | Mouse genotyping | AGAAAATGCCAGGATGATGC | TGTCATCAGGGACTTTGCTG | 164 |
| TLR4 | Mouse | TTTATTCAGAGCCGTTGGTG | CAGAGGATTGTCCTCCCATT | 186 |
| TLR4- ko | Mouse genotyping | CAGCAAAGTCCCTGATGACA | TCCAGCCACTGAAGTTCTGA | 117 |
| TNF-α | Mouse | TTCCGAATTCACTGGAGCCTCGAA | TGCACCTCAGGGAAGAATCTGGAA | 144 |
| RPLO | Human/Mouse | GGCGACCTGGAAGTCCAACT | CCATCAGCACCACAGCCTTC | 143 |