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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: J Immunol. 2016 Jun 24;197(3):923–933. doi: 10.4049/jimmunol.1502611

Figure 6. The CLD of cFLIPL is sufficient and necessary to co-immunoprecipitate with IRF3 and to prevent CBP-IRF3 interactions.

Figure 6

Immunoblot (IB) analysis of (A) GFP-IRF3 fusion protein immunoprecipitated (IP) from 293T cells co-transfected with pGFP-IRF3 (500 ng), pCBP (500 ng) and various combinations of pCI, pTBK1 and FLAG-tagged cFLIP proteins. Anti-GFP was used as the antibody in these IPs. IP samples were probed for the presence of CBP and FLAG-tagged FLIP proteins by immunoblotting (IB). (B) A portion of each lysate prior to immunoprecipitation was set aside as input sample, and immunoblotted to detect protein expression. (C) IB analysis of proteins IP-ed from cellular lysates with non-specific IgG instead of anti-GFP. All experiments were performed at least three times.