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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: J Immunol. 2016 Jun 24;197(3):923–933. doi: 10.4049/jimmunol.1502611

Figure 7. Endogenous IRF3 interacts with cFLIPL and the CLD, but not cFLIPS.

Figure 7

Immunoblot (IB) analysis of an immunoprecipitated (IP) endogenous IRF3 from 293T cells co-transfected with plasmids encoding FLAG-tagged cFLIP proteins and various combinations of pCI and pTBK1 or pMAVS. (A) Cellular lysates were IP-ed with (A) anti-IRF3 or (B) anti-FLAG antibodies and IP-ed samples were probed for the presence of IRF3 and FLAG-tagged FLIP proteins by immunoblotting. (C and D) A portion of each lysate prior to immunoprecipitation was set aside as input sample, and immunoblotted to detect protein expression. (E and F) IB analysis of proteins IP-ed with non-specific IgG instead of anti-IRF3 or anti-FLAG. All experiments were performed at least three times.