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Electron micrographs of aberrant polymer formed with vinblastine in the absence (A) and presence of GTP (B). Reaction mixtures contained 10 μM tubulin, 0.75 M monosodium glutamate, 20 μM vinblastine, and, if present, 10 μM GTP. The tubulin used in these studies was not subjected to gel filtration chromatography.
