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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: Arch Biochem Biophys. 2016 Jun 13;604:63–73. doi: 10.1016/j.abb.2016.06.008

Fig. 6.

Fig. 6

Concentration effects of (−)-rhazinilam and NSC 613241 on amount of tubulin in sedimentable polymer (A) and on stoichiometry of compounds in sedimentable polymer (B). Experimental details are described in detail in the text. In brief, reaction mixtures containing the indicated concentrations of [3H](−)-rhazinilam (triangles) or [3H]NSC 613241 (circles) were incubated at 22 °C for 30 min, and the polymer was harvested by centrifugation. Protein and radiolabel content of the pellets was determined, and these data were used to calculate the values shown in the Figure.