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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: Arch Biochem Biophys. 2016 Jun 13;604:63–73. doi: 10.1016/j.abb.2016.06.008

Fig. 7.

Fig. 7

Both (−)-rhazinilam and NSC 613241 are required for aberrant assembly at 0 °C in the absence of GTP. Reaction mixtures contained 10 μM tubulin, 0.6 M monosodium glutamate (pH 6.6), 1.0 mM MgCl2, 4% dimethyl sulfoxide, and NSC 613241 and/or (−)-rhazinilam, as indicated. A. Aberrant polymer harvested by ultracentrifugation as described in the text following a 20 min incubation at 0 °C. Symbols: circles, (−)-rhazinilam and NSC 613241 at the indicated concentrations; upright triangles, (−)-rhazinilam at the indicated concentrations; inverted triangles, NSC 613241 at the indicated concentrations. B. Formation of aberrant polymer by turbidimetry. Reactions were followed at 0 °C in a Gilford 250 spectrophotometer equipped with an electronic temperature controller. Curve 1, 15 μM (−)-rhazinilam and 15 μM NSC 613241; curve 2, 15 μM (−)-rhazinilam; curve 3, 30 μM (−)-rhazinilam; curve 4, 15 μM NSC 613241; curve 5, 30 μM NSC 613241.