Table 3.

(−)-Rhazinilam and NSC 613241 do not appear to affect each other’s binding to aberrant polymer^a

Radiolabeled ligand	Potential inhibitor	Ligand bound/tubulin in aberrant polymer ± SD (% tubulin recovered in pellet)
10 μM (−)-Rhazinilam		0.37 ± 0.053 (37 ± 5.1)
10 μM (−)-Rhazinilam	80 μM NSC 613241	0.32 ± 0.022 (89 ± 6.4)
10 μM NSC 613241		0.15 ± 0.013 (72 ± 5.4)
10 μM NSC 613241	80 μM (−)-rhazinilam	0.16 ± 0.014 (95 ± 5.2)

^aExperimental conditions were as described in the text. In brief, reaction mixtures containing the indicated concentrations of [³H](−)-rhazinilam, [³H]NSC 613241, nonradiolabeled NSC 613241, or nonradiolabeled (−)-rhazinilam were incubated at 22 °C for 30 min, and the polymer was harvested by centrifugation. Protein and radiolabel content of the pellets was determined, and these data were used to calculate the values shown in the Table.