. Author manuscript; available in PMC: 2017 Aug 15.

Published in final edited form as: Arch Biochem Biophys. 2016 Jun 13;604:63–73. doi: 10.1016/j.abb.2016.06.008

Table 4.

Both a DPP and (−)-rhazinilam or a rhazinilam analogue are required for polymer formation without GTP at 0 °C^a

Compound(s) added	% tubulin in polymer ± SE
(−)-Rhazinilam^b	0 ± 2
Racemic rhazinilam analogue^c	0 ± 3
NSC 613241^b	0 ± 2
NSC 608593^b	0 ± 1
(−)-Rhazinilam^d; racemic rhazinilam analogue^c	0 ± 2
NSC 613241^d; NSC 608593^d	0 ± 2
(−)-Rhazinilam^d; NSC 613241^d	69 ± 3.0
(−)-Rhazinilam^d; NSC 608593^d	72 ± 1.4
Racemic rhazinilam analogue^b; NSC 613241^d	17 ± 1.9
Racemic rhazinilam analogue^b; NSC 608593^d	19 ± 3.8

Reaction mixtures contained 1.0 mg/mL tubulin, 0.6 M monosodium glutamate (pH 6.6), 1.0 mM MgCl₂, compounds at the indicated concentrations, and 4% dimethyl sulfoxide. Incubation was for 20 min at 0 °C, with centrifugation as described in the text. The protein concentration in the supernatants was compared to the protein concentration in uncentrifuged samples to determine the % tubulin in the polymer pellets.

Compound concentrations were 30 μM.

Compound concentrations were 60 μM.

Compound concentrations were 15 μM.