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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Innate Immun. 2016 May 26;22(5):363–372. doi: 10.1177/1753425916650027

Figure 5. rIFN-β does not enhance TLR2 expression or cytokine gene induction in response to purified TLR2 ligands.

Figure 5

TEPM were treated either with media alone or 100 U/ml rIFN-β for 4 h. (A) Total RNA from media and rIFN-β-treated macrophages was harvested and used in RT-PCR analysis of steady-state TLR2 transcript levels. (B) Media and rIFN-β-treated macrophages were fixed and stained for TLR2 surface expression by flow cytometry. (C) Quantitation of mean fluorescence intensity from samples analyzed in (B). (D and E) Media- and rIFN-β pre-treated macrophages were stimulated for 3 h with the indicated dose of TLR2 ligand P3C (D) or P2C (E). Following stimulation total RNA was harvested and used for RT PCR analysis of IL-6 or TNFα transcript levels. Results are represented as mean ± SEM from 3 experiments. (F) Media and rIFN-β-treated macrophages were stimulated with 250 ng/ml P3C for indicated times and cell lysates probed utilizing indicated antibodies.