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. Author manuscript; available in PMC: 2016 Jul 21.
Published in final edited form as: Methods Enzymol. 2012;506:361–389. doi: 10.1016/B978-0-12-391856-7.00042-1

Figure 18.1.

Figure 18.1

Development of (A) preimplantation and (B) postimplantation embryos. Requirements for dissection and culture media as well as gas content are shown for each embryonic stage. NCS, newborn calf serum; RS, rat serum; E, embryonic day (adapted from Nowotschin et al., 2010; with permission from Academic Press/Elsevier).