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. Author manuscript; available in PMC: 2017 Aug 26.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Jun 21;477(3):363–368. doi: 10.1016/j.bbrc.2016.06.099

Figure 2. Comparison of cell line 85 h2E2 mAb samples prepared following the protocol outlined in Figure 1 using TBP or TCEP as the reductant.

Figure 2

Cell line 85 mAb samples were prepared for mass spectral analyses, either using the protocol delineated in Figure 1, or using a modification of the Figure 1 protocol with TCEP replacing TBP as the reductant. Multiple aliquots of the resulting samples were analyzed by 10% SDS-PAGE, without DTT reduction prior to electrophoresis. The gel was subsequently stained with Coomassie blue.