Table 1.
Theoretical vs experimental masses for the h2E2 mAb glycoforms detected and quantified (see Figure 3B for experimental masses)
| Fc/2 fragment glycoform peak | Theoretical mass (Da) | Experimental mass (Da) |
|---|---|---|
| Man5 | 25235.4 | 25235.4 |
| G0 | 25316.9 | 25317.9 |
| G0F | 25463.6 | 25464.5 |
| G0F + K | 25591.8 | 25592.7 |
| G1F | 25625.7 | 25626.5 |
| G1F + K | 25753.9 | 25754.6 |
| G2F | 25787.8 | 25788.6 |
| G2F + K | 25916.0 | 25918.0 |
The theoretical masses were calculated starting with the calculated theoretical mass for the main peak, G0F = 25463.6 (calculated in the text of the Results section), and then using; fucose added/subtracted mass = 146.1, hexose added mass = 162.1, and lysine added mass = 128.2