. Author manuscript; available in PMC: 2017 Jul 19.

Published in final edited form as: Biochemistry. 2016 Jul 11;55(28):3864–3873. doi: 10.1021/acs.biochem.6b00113

Table 2.

¹⁹F Resonance frequencies and linewidths^a

	RT127tfmF		RT146tfmF		RT181tfmF		RT181tfmF- V108I		RT181tfmF- E138K(p51)		RT181tfmF-K103N
	ppm	Hz	ppm	Hz	ppm	Hz	ppm	Hz	ppm	Hz	ppm	Hz
Apo	−61.5	100 ± 2	−60.2 −62.0	150 ± 85 150 ± 5	−60.8^b	500 ± 5	−61.1^b	500 ± 5	−60.8^b	600 ± 20	−60.1	300 ± 15
EFV	−61.5	100 ± 5	−60.2 −61.8	180 ± 1 180 ± 2	−60.3	125 ± 2	−60.3	125 ± 5	−60.2	170 ± 5	−60.4	150 ± 2
ETR					−60.3	125 ± 2	−60.3	125 ± 5	−60.2	125 ± 2	−60.4	145 ± 2
RPV					−60.7	140 ± 2	−60.7	125 ± 5	−60.7	125 ± 5	−60.7	145 ± 2
NVP			−60.2 −61.9^b	180 ± 30 200 ± 25	−59.7 −60.8^b	180 ± 5 500 ± 10	−59.7 −61.1^b	125 ± 10 400 ± 10	−59.7 −60.9^b	125 ± 10 600 ± 15	−60.1^b	300 ± 20

the upper limit of uncertainty in the linewidth was qualitatively estimated as described in Materials and Methods.

although the signal is not symmetric, the resonance frequency and the linewidth were extracted assuming a single peak.