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. Author manuscript; available in PMC: 2017 Jul 19.
Published in final edited form as: Biochemistry. 2016 Jul 11;55(28):3864–3873. doi: 10.1021/acs.biochem.6b00113

Table 3.

19F Resonance frequencies and linewidths of mutants of RT146tfmFa

RT146tfmF-
V108I 		RT146tfmF-
K103N
ppm Hz ppm Hz
Apo −60.1
−62.1		 150 ± 30
150 ± 5		 −60.1
−62.1		 150 ± 30
150 ± 5
EFV −60.1
−61.9b 		180 ± 5
300 ± 15		 −60.1
−61.9b 		180 ± 5
260 ± 5
NVP −60.2
−61.9b 		180 ± 10
200 ± 25		 −60.1
−62.1		 150 ± 30
150 ± 5
a

the upper limit of uncertainty in the linewidth was qualitatively estimated as described in Materials and Methods.

b

although the signal is not symmetric, the resonance frequency and linewidth extracted assuming a single peak..