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VPA significantly increases the expression of Snail. (A) HCT-116 or SW480 cells were treated with 1 mM VPA for 24 h, and then the expression of Snail, Slug, Zeb, Twist were measured by use of Western blot analysis; (B) HCT-116 cells were treated with 1 mM VPA for 24 h, the cellular location of Snail (green) were examined by immunofluorescence staining and nuclei were stained with DAPI (blue).
