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. 2015 Nov 10;9(6):502–512. doi: 10.1080/19336918.2015.1113366

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — Staurosporine-induced ERM dephosphorylation and cell adhesion in KG-1 cells. (A) Staurosporine (Sta) treatment-induced adhesion of KG-1 cells. KG-1 cells were incubated in the GST-CS1-coated plate with or without Staurosporine. Images of cells remained attached to plate after wash are demonstrated. Magnification: x40. Scale bar: 400 μm. (B) Effect of Staurosporine on cell adhesion. Unattached cells in the adhesion assay were counted and compared. Ratios of unattached cells per total incubated cells are demonstrated by percentage. Treatment with 1nM or more Staurosporine decreased unattached cells. Concentration: nM (C) Immunoblot analysis with anti-phospho-ERM Ab. Lysates of Staurosporine-treated or untreated KG-1 cells were analyzed by immunoblotting. Concentrations of Staurosporine (nM) are indicated. (D) Immunofluorescent analysis with anti-Ezrin Ab. KG-1 cells attached to poly-L-lysine-coated coverglass chamber treated with or without 10 nM Staurosporine were analyzed by immunocytochemistry. Staining with anti-Ezrin Ab was detected at the edge of cells in untreated cells, while detected at the cytoplasm in Staurosporine-treated cells. Magnification: x200. Scale bar: 100 μm.