Table 2.
Changes in some identified genes that are highly relevant to pain, itch, touch, thermal, and chemical sensitivity in the injured DRG after SNL.
| Upregulation |
Downregulation |
||||
|---|---|---|---|---|---|
| Name | Description | Ratio change | Name | Description | Ratio change |
| Agtr2 | Angiotensin II receptor, type 2 | 25.34 | Adra2c | Adrenergic receptor, alpha 2 c | 0.09 |
| Aqp9 | Aquaporin 9 | 4.35 | Asic3 | Acid-sensing (proton-gated) ion channel 3 | 0.39 |
| Atf3 | Activating transcription factor 3 | 9.64 | Atp1a1 | ATPase, Na+/K+ transporting, alpha 1 polypeptide | 0.27 |
| Cacna1d | Calcium channel, voltage-dependent, L type, alpha 1D subunit | 2.30 | Atp1b3 | ATPase, Na+/K+ transporting, beta 3 polypeptide | 0.63 |
| Cacna1g | Calcium channel, voltage-dependent, T type, alpha 1 G subunit | 2.18 | Calca | Calcitonin/calcitonin-related polypeptide, alpha | 0.14 |
| Cacna1h | Calcium channel, voltage-dependent, T type, alpha 1 H subunit | 1.05 (n.s.) | Chrna4 | Cholinergic receptor, nicotinic, alpha polypeptide 4 | 0.12 |
| Cacna2d1 | Calcium channel, voltage-dependent, alpha2/delta subunit 1 | 3.07 | Chrna6 | Cholinergic receptor, nicotinic, alpha polypeptide 6 | 0.01 |
| Cacng3 | Calcium channel, voltage-dependent, gamma subunit 3 | 2.31 | Chrnb3 | Cholinergic receptor, nicotinic, beta polypeptide 3 | 0.02 |
| Cckbr | Cholecystokinin B receptor | 10.00 | Gabbr1 | Gamma-aminobutyric acid (GABA) B receptor, 1 | 0.40 |
| Ccr1 | Chemokine (C-C motif) receptor 1 | 5.67 | Gabra1 | Gamma-aminobutyric acid (GABA) A receptor, alpha 1 | 0.07 |
| Ccr3 | Chemokine (C-C motif) receptor 3 | 8.25 | Gabrg2 | Gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 | 0.10 |
| Ccr5 | Chemokine (C-C motif) receptor 5 | 7.05 | Galr1 | Galanin receptor 1 | 0.05 |
| Chrna5 | Cholinergic receptor, nicotinic, alpha polypeptide 5 | 3.25 | Gria2 | Glutamate receptor, ionotropic, AMPA2 (alpha 2) | 0.11 |
| Gal | Galanin | 10.06 | Grik1 | Glutamate receptor, ionotropic, kainate 1 | 0.05 |
| Grin2a | Glutamate receptor, ionotropic, N-methyl D-aspartate 2 A | 6.64 | Htr1a | 5-Hydroxytryptamine (serotonin) receptor 1 A | 0.04 |
| Htr2b | 5-Hydroxytryptamine (serotonin) receptor 2B | 35.49 | Htr2c | 5-Hydroxytryptamine (serotonin) receptor 2 C | 0.14 |
| Hvcn1 | Hydrogen voltage-gated channel 1 | 5.90 | Htr3a | 5-Hydroxytryptamine (serotonin) receptor 3a | 0.01 |
| Kcnh4 | Potassium voltage-gated channel, subfamily H (eag-related), member 4 | 4.02 | Htr3b | 5-Hydroxytryptamine (serotonin) receptor 3b | 0.02 |
| Kcnj15 | Potassium inwardly rectifying channel, subfamily J, member 15 | 7.43 | Htr4 | 5-Hydroxytryptamine (serotonin) receptor 4 | 0.04 |
| Kcnk13 | Potassium channel, subfamily K, member 13 | 2.74 | Htr5b | 5-Hydroxytryptamine (serotonin) receptor 5b | 0.08 |
| Kcnk16 | Potassium channel, subfamily K, member 16 | 6.80 | Htr7 | 5-Hydroxytryptamine (serotonin) receptor 7 | 0.44 |
| Kcnk6 | Potassium inwardly rectifying channel, subfamily K, member 6 | 3.84 | Kcnb2 | Potassium voltage gated channel, Sham-related subfamily, member 2 | 0.10 |
| Mcoln2 | Mucolipin 2 | 9.84 | Kcnc2 | Potassium voltage gated channel, Sham-related subfamily, member 2 | 0.78 (n.s.) |
| P2rx1 | Purinergic receptor P2X, ligand-gated ion channel, 1 | 3.13 | Kcng3 | Potassium voltage-gated channel, subfamily G, member 3 | 0.06 |
| P2rx4 | Purinergic receptor P2X, ligand-gated ion channel 4 | 1.97 | Kcng4 | Potassium voltage-gated channel, subfamily G, member 4 | 0.11 |
| P2rx7 | Purinergic receptor P2X, ligand-gated ion channel, 7 | 2.26 | Kcnj4 | Potassium inwardly rectifying channel, subfamily J, member 4 | 0.10 |
| P2ry13 | Purinergic receptor P2Y, G-protein coupled 13 | 6.52 | Kcnk18 | Potassium channel, subfamily K, member 18 | 0.04 |
| P2ry6 | Pyrimidinergic receptor P2Y, G-protein coupled, 6 | 6.07 | Kcnmb1 | Potassium large conductance calcium-activated channel, subfamily M, beta member 1 | 0.03 |
| Pkd2l1 | Polycystic kidney disease 2-like 1 | 6.26 | Kcns1 | Potassium voltage-gated channel, subfamily S, 1 | 0.06 |
| Tacr1 | Tachykinin receptor 1 | 15.43 | Kcnt1 | Potassium channel, subfamily T, member 1 | 0.13 |
| Tpcn2 | Two pore segment channel 2 | 2.28 | Kcnv1 | Potassium channel, subfamily V, member 1 | 0.11 |
| Trpm1 | Transient receptor potential cation channel, subfamily M, member 1 | 3.43 | Mrgpra1 | MAS-related G protein-coupled receptors, member A1 | 0.03 |
| Trpm5 | Transient receptor potential cation channel, subfamily M, member 5 | 2.05 | Mrgpra2a | MAS-related G protein-coupled receptors, member A2A | 0.12 |
| Trpv4 | Transient receptor potential cation channel, subfamily V, member 4 | 1.89 | Mrgpra2b | MAS-related G protein-coupled receptors, member A2B | 0.03 |
| Mrgpra3 | MAS-related G protein-coupled receptors, member A3 | 0.01 | |||
| Mrgprd | MAS-related G protein-coupled receptors, member D | 0.01 | |||
| Mrgprx1 | MAS-related G protein-coupled receptors, member X1 | 0.02 | |||
| Oprd1 | Opioid receptor, delta 1 | 0.08 | |||
| Oprk1 | Opioid receptor, kappa 1 | 0.02 | |||
| Oprl1 | Opioid receptor-like 1 | 0.09 | |||
| Oprm1 | Opioid receptor, mu 1 | 0.09 | |||
| P2ry1 | Purinergic receptor P2Y, G-protein coupled 1 | 0.32 | |||
| Piezo2 | Piezo-type mechanosensitive ion channel component 2 | 0.71 | |||
| Scn10a | Sodium channel, voltage-gated, type X, alpha | 0.05 | |||
| Scn11a | Sodium channel, voltage-gated, type XI, alpha | 0.04 | |||
| Scn1a | Sodium channel, voltage gated, type I alpha subunit | 0.09 | |||
| Sst | Somatostatin | 0.02 | |||
| Sstr2 | Somatostatin receptor 2 | 0.05 | |||
| Trpa1 | Transient receptor potential cation channel, subfamily A, member 1 | 0.04 | |||
| Trpm8 | Transient receptor potential cation channel, subfamily M, member 8 | 0.06 | |||
| Trpv1 | Transient receptor potential cation channel, subfamily V, member 1 | 0.33 | |||
| Vip | Vasoactive intestinal polypeptide | 0.70 (n.s.) | |||
SNL: spinal nerve ligation; DRG: dorsal root ganglia. All ratio changes are significant p < 0.05 except if indicated by “n.s.”—no significant.