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. 2016 Jun 17;12:1744806916648153. doi: 10.1177/1744806916648153

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© The Author(s) 2016

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Perfusion of high dose DA inhibits ACC pyramidal eEPSCs. Voltage-clamp whole-cell recordings of eEPSCs in pyramidal neurons of layer II/III of mice ACC. (a) Representative diagram of recording location at layer II/III and stimulation of layer V/IV in mice ACC, image credit: Allen Institute, mouse coronal brain atlas, version 1:2008, coronal level 43. (b) Sample action potential firing pattern of pyramidal neuron in current-clamp mode by injection of 15 mV currents for 400 ms, every 15 s. (c) Sample EPSC trace of AMPA/KA currents in layers II/III, depicting the time course for reduction of EPSC amplitude due to DA interaction with DARs and recovery after washing out DA. The AMPA/KAR currents were recorded in presence of PTX (100 μM). (d) Averaged and normalized (%) of eEPSCs (n = 11/8 mice). (e) Inhibition induced by DA (50 μM) was statistically significant in comparison to the baseline and washout (one-way ANOVA, ***P < 0.001). (f) Averaged and normalized (%) of eEPSCs showing no inhibition in response to DA (5 μM; blue, n = 5/4 mice). (g) Results for statistical analysis of DA (5 μM) against baseline (t-test, P = 0.65), data shown in comparison to DA (50 μM). Statistical analyses were performed for 5 min bins. Error Bars represent SEM. eEPSCs: evoked excitatory postsynaptic currents.