Figure 5.

Inhibition of PLCγ but not PI3K/Akt attenuated colitis-induced CGRP expression in L1 DRG. Animals were induced for colitis followed by treatment with either vehicle (A, D), or specific inhibitors against PLC (B: U73122) or PI3K (E: LY294002). CGRP immunoreactivity was examined in L1 DRG from all animals. Colitis-induced CGRP expression was attenuated by inhibition of the PLCγ pathway (C). However, suppression of the endogenous PI3K activity with LY294002 had no effect on CGRP expression level evoked by colitis (F). Bar = 80 µm. *p < 0.05. n = 3 animals for each group.