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. 2016 May 23;65(8):2235–2248. doi: 10.2337/db15-1443

Figure 4.

Figure 4

IRS2 levels are increased and downstream pathways are activated in Eif4ebp2-deficient islets. A: Immunoblotting (left) and quantification (right) of IRS2, IRS1, phospho-Erk, phospho-Creb, phospho-Akt, and mTORC1 targets (pS6k and pS6) using islet lysates from wild-type (WT; white bars) and Eif4ebp2−/− (black bars) mice. B: Immunoblotting for IRS2 and tubulin using islet lysates from WT, Eif4ebp1−/−, and Eif4ebp2−/− mice. C: Assessment of 4ebp2, Irs2, and Irs1 mRNA levels in islets lysates from WT and Eif4ebp2−/− mice using TaqMan RT-PCR. Data are shown as mean ± SEM (n = 4 mice per group). *P < 0.05.