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. 2016 May 23;65(8):2235–2248. doi: 10.2337/db15-1443

Figure 7.

Figure 7

Stability of IRS2 depends on Jak2 activation. A: IRS2 protein stability assessed by immunoblotting (left) and quantification (right) for IRS2 and tubulin in 4E-BP2kd (gray line) and control cells (black line) cultured with 12.5 μg/mL CHX and AG490 for 0, 2, 6, and 8 h. B: IRS2 protein stability assessed by immunoblotting (left) and quantification (right) for IRS2 and tubulin in Eif4ebp2−/− and wild-type (WT) islets cultured with 12.5 μg/mL CHX and AG490 for 0 and 8 h (samples were run in the same gel but appear spliced to show WT data to the left of Eif4ebp2−/−). C: Immunoblotting for IRS2 and tubulin in dispersed islets from Eif4ebp2−/− (control or with IRS2 silenced) (left). Apoptotic rate (middle) and images (right) in Eif4ebp2−/− dispersed cells (control or with IRS2 silenced) treated with cytokines for 24 h. Data are shown as mean ± SEM (n = 4 mice per group). *P < 0.05. a.u., arbitrary units.