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. 2016 May 23;65(8):2331–2341. doi: 10.2337/db15-1527

Figure 4.

Figure 4

Altered cell cycle regulators and serotonin signaling in βPRLRKO islets during pregnancy. mRNA levels of Prlr and Cish (A) and Tph1 (B) in nonpregnant (N) or pregnant (P) βPRLRKO females (black bars) and controls (open bars). C: Immunofluorescence of islet serotonin, insulin, and DAPI (scale bar, 50 μm). D: Relative mRNA levels of Ccna2, Ccnb1, Ccnb2, Ccnd1, Ccnd2, and Foxm1 in βPRLRKO females and controls at GD16.5. E: Confocal immunofluorescence images of islets from GD16.5 βPRLRKO females and controls (original magnification ×40). MafB (red), insulin (green), and DAPI (blue) with MafB+Ins+ cells highlighted (white arrows). For mRNA studies, pregnant samples were from GD16.5 and expression levels normalized to nonpregnant controls = 1. n = 3–8 mice/group. *P < 0.05, **P ≤ 0.01 by t test.