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. 2016 May 23;65(8):2367–2379. doi: 10.2337/db15-1607

Figure 1.

Figure 1

Generation of c-Rel–deficient NOD mice. A: c-Rel knockout mice in C57BL/6 background were mated with NOD mice, and the heterozygotes obtained were mated with NOD mice in six successive generations as indicated in the table. B: Up to 15 mice from each generation were assessed by genome scanning to identify the percent incorporation of NOD genome. Shown is the highest percentage of NOD genome obtained in each generation. C and D: Expression of NF-κB family members in thymus and spleen of c-Rel–deficient C57BL/6 and NOD mice. Total cell lysates of thymus (C) and spleen (D) of 6–8-week-old mice were prepared and 20–30 μg of protein probed as indicated. Two representative samples for each strain are shown from four or more mice per strain. Normalized protein levels were confirmed by β-actin staining.