Table 3. The number of private genes during ontogeny in C. maculatus.

		All	ORFs	Blast Nr	ORFs with BlastNr
Genes		145,883	27,878	33,129	22,401
	FPKM> = 2	212	51	51	38
Larvae	2>FPKM> = 0.5	1,623	114	179	80
	2>FPKM>0	8,453	895	1,288	628
	FPKM> = 2	531	64	63	35
Pupae	2>FPKM> = 0.5	2,946	159	266	101
	2>FPKM>0	14,823	1,437	2,197	1,017
	FPKM> = 2	455	222	171	151
Adults	2>FPKM> = 0.5	2,365	367	447	283
	2>FPKM>0	16,669	2,196	2,894	1,593

Here, private genes are defined as those expressed at low (either 2>FPKM >0 or 2>FPKM> = 0.5) or higher levels (FPKM> = 2) in a particular developmental stage, but not found expressed in any of the other stages (FPKM = 0).