Skip to main content
. 2016 Jul 21;11(7):e0159346. doi: 10.1371/journal.pone.0159346

Fig 2. Darkness-dependent regulation of lrtA mRNA and protein levels.

Fig 2

A, Total RNA was isolated from early-log-phase Synechocystis cells growing under normal illumination conditions (Light), after being subjected to darkness for 15, 30 or 60 min, and after re-illumination of the culture for 15 or 30 min. 15 μg of total RNA was loaded per lane. Levels of lrtA and glnA mRNA were determined by Northern blotting. All the filters were stripped and re-hybridized with a rnpB gene probe as loading control. The data shown are representative of four independent Northern-blot experiments showing similar results. B, Level of LrtA protein was determined by Western blotting using anti-LrtA serum. 6 μg of total protein was loaded per lane. The data shown are representative of three independent Western-blot experiments showing similar results.